光独立栄養培養におけるChlorella sp. UK001の細胞径変化に及ぼす培養温度の効果

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  • Effect of Temperature on Change in Cell Diameter of Chlorella sp. UK001 in Photoautotrophic Culture.
  • ヒカリ ドクリツ エイヨウ バイヨウ ニ オケル Chlorella sp.U

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Chlorella sp. UK 001 cells were cultivated under a photoautotrophic condition, utilizing carbon dioxide and light as carbon and energy sources, respectively. In batch cultures of the cells for 96 h at incident light intensity (I0) of 21.8 W m-2, when the culture temperature (T) was kept at 40°C, mean diameter of the cells (dc) increased from 2.3 μm (standard deviation (SD) =0.36 μm) to 11 μm (SD = 1.9 μm), while at T = 20, 30, 35 and 45°C, the values of de hardly changed. Under the conditions examined, the appreciable enlargement of the cells occurred at T =40°C and at I0 = 16 to 46 W m-2, and was not accompanied by a substantial increase in the number of cells. From the data on cell viability, oxygen generation rate under light conditions and oxygen consumption rate under dark conditions, the enlarged cells in the culture at 40° C were found to have sufficient activities as living cells. Sedimentation experiments were carried out by standing the cells which were cultivated at 30 and 40°C. The interfacial sedimentation rate of the cells (dc= 11 μm, SD= 1.9 μm) obtained from the culture at 40°C was about twelve times higher than that of cells (de = 2.3 μm, SD = 0.38μm) at 30°C

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