Application of Novel Sensors for Bioprocess On-Line Monitoring to Dextran Fermentation.
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- Endo Hideaki
- RCAST, Univ. of Tokyo
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- Suzuki Masayasu
- RCAST, Univ. of Tokyo
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- Sode Koji
- RCAST, Univ. of Tokyo
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- Tamiya Eiichi
- RCAST, Univ. of Tokyo
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- Karube Isao
- RCAST, Univ. of Tokyo
Bibliographic Information
- Other Title
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- バイオプロセス用センサーによるデキストラン発酵のオンラインモニタリング
- バイオプロセスヨウ センサー ニ ヨル デキストラン ハッコウ ノ オンライン
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Abstract
An on-line monitoring system was developed for the measurement of various parameters such as cell concentration, viscosity of broth and sucrose concentration in dextran fermentation. This system was constructed from a piezoelectric gum sensor, a quartz crystal viscous sensor, a sucrose sensor, a bioreactor and a personal computer.<BR>A piezoelectric gum sensor for the determination of biomass was prepared with two piezoelectric gums, a pulse generator and an amplifier. The calibration curve for L. mesenteroides was linear in the range of 1.0-4.8 g dry cell mass.l-1. A quartz crystal viscous sensor was prepared with AT-cut quartz crystal, an oscillating circuit and a peak level meter. A linear relationship was obtained between resonant resistance (R1) of the quartz crystal and (ρη) 1/2 of dextran solution, where ρ and η were density and viscosity respectively. A sucrose sensor was prepared with three immobilized enzyme layers (glucose oxidase, mutarotase and invertase) and a gold working electrode. The calibration curve for sucrose was linear up to 1000 mg·l-1.<BR>An on-line monitoring system was applied to dextran fermentation, and these paramenters were measured. Good correlation was obtained between the sensor signals and the values determined by conventional methods.
Journal
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- KAGAKU KOGAKU RONBUNSHU
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KAGAKU KOGAKU RONBUNSHU 17 (3), 559-564, 1991
The Society of Chemical Engineers, Japan
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Details 詳細情報について
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- CRID
- 1390001204509426048
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- NII Article ID
- 130000871210
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- NII Book ID
- AN00037234
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- ISSN
- 13499203
- 0386216X
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- NDL BIB ID
- 3719132
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed