Bioseparation Engineering. Mathematical Modeling of Continuous Separation Process of Proteins using Partial Recycling Free-Flow Isoelectric Focusing with Feed Supply and Product Withdrawal.

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  • 生物分離工学　　部分循環式フリーフロー等電点電気泳動法による二成分タンパク質の連続分離プロセスに関する数学モデル
  • 部分循環式フリーフロー等電点電気泳動法による二成分タンパク質の連続分離プロセスに関する数学モデル
  • ブブン ジュンカンシキ フリーフロー トウデンテン デンキ エイドウホウ ニ ヨル 2セイブン タンパクシツ ノ レンゾク ブンリ プロセス ニ カンスル スウガク モデル

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An unsteady state mathematical model describing the continuous separation process of a binary mixture of protein using partial recycling free-flow isoelectric focusing is constructed. The model consists of a one-dimensional mass balance equation and an electric charge balance equation for the electrophoretic chamber and a mass balance equation for the reservoir. The model equations were solved numerically using a finite difference method with literature values of physical constants such as dissociation and diffusion constants. The calculated results are in good agreement with the experimental data for the separation of protein mixture of bovine serum albumin (BSA) and bovine hemoglobin (Hb) reported previously. Furthermore, the model can describe the effects of the operating factors, such as support electrolyte concentration and feed flow rate, on the separation process.

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