楕円ローター型粉砕機を用いた菌体破砕と細胞内成分の放出制御

書誌事項

タイトル別名
  • The Disruption of Yeast Cells and the Release Control of Cell Contents Using a Mill with an Elliptic Rotor.
  • ダエン ローターガタ フンサイキ オ モチイタ キンタイ ハサイ ト サイボウ

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抄録

The Theta-composer is a new type of equipment developed for preparing composites of various particulate materials. In this study, yeast cells were disintegrated by a theta-composer using glass beads 1.0mm in diameter as the disruption medium. The efficiency of disruption was determined by the degree of released soluble protein. In this study, invertase (β-D-fructofuranoside fructohydrolase), ADH (alcohol dehydrogenase) and G-6-PDH (D-glucose-6-phosphate dehydrogenase) were assessed as representatives of the cell wall-bounded enzyme, the enzymes around the cell wall and cytoplasmic enzyme, respectively.<br>The rotor speed of the theta-composer was the specific factor influencing the degree of disruption. ADH and invertase were released faster than G-6-PDH. These results suggest that the theta-composer is effective in the selective release of the components from yeast cell by controlling the degree of disruption.

収録刊行物

  • 粉体工学会誌

    粉体工学会誌 34 (9), 666-671, 1997

    一般社団法人 粉体工学会

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