Screening of IgG-Fc Binding Peptides from Milk Protein Using Slide Glass Type-Exclusive Peptide Array
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- Katayama Makoto
- Department of Biotechnology, Graduate School of Engineering, Nagoya University
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- Sugita Tomoya
- Department of Biotechnology, Graduate School of Engineering, Nagoya University
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- Kato Ryuji
- Department of Biotechnology, Graduate School of Engineering, Nagoya University
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- Okochi Mina
- Department of Biotechnology, Graduate School of Engineering, Nagoya University
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- Matsushima Miyoko
- School of Health Sciences, Nagoya University
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- Kawabe Tsutomu
- School of Health Sciences, Nagoya University
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- Takase Tomokazu
- R&D Center, NGK Insulators, Ltd.
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- Yoshida Yasuko
- R&D Center, NGK Insulators, Ltd.
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- Kawase Mitsuo
- R&D Center, NGK Insulators, Ltd.
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- Honda Hiroyuki
- Department of Biotechnology, Graduate School of Engineering, Nagoya University
Bibliographic Information
- Other Title
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- ミルクタンパク質全網羅ペプチドアレイを用いたIgG結合ペプチドの探索
- ミルクタンパクシツ ゼン モウラ ペプチドアレイ オ モチイタ IgG ケツゴウ ペプチド ノ タンサク
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Abstract
IgG–Fc binding peptide which functions as ligand for IgG purification was screened by peptide array. When sera from milk allergy patient were applied to the milk protein-explosive peptide array, some 16-mer binding peptides were found from both positive sera and negative sera. When cellulose membrane type peptide array was used for identification of binding peptide sequence, octamer peptide, LLRLKKYK, was found to strongly bind to mouse IgG. Since the octamer peptide weakly bound with IgA, it was suggested that the peptide is an IgG–Fc specific binding peptide. Furthermore, it was also suggested that the IgG-Fc binding peptide is available for purification of IgG–Fc.
Journal
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- KAGAKU KOGAKU RONBUNSHU
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KAGAKU KOGAKU RONBUNSHU 37 (6), 546-550, 2011
The Society of Chemical Engineers, Japan
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Details 詳細情報について
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- CRID
- 1390001204513314944
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- NII Article ID
- 10030160249
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- NII Book ID
- AN00037234
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- ISSN
- 13499203
- 0386216X
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- NDL BIB ID
- 023454105
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed