Sustained expression of dwarf symptoms in mulberry shoots grown in vitro and their disappearance through the culture of multiple bud bodies.

DOI JASI Web Site 14 References
  • OKA SEIBI
    National Institute of Agrobiological Resources Present address: Plant-Biotechnology Institute, Ibaraki Agricultural Center
  • YAMANOUCHI HIROAKI
    National Institute of Sericultural and Entomological Science
  • KAWAKITA HIROMU
    National Institute of Sericultural and Entomological Science
  • MACHII HIROAKI
    National Institute of Sericultural and Entomological Science

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Other Title
  • クワ萎縮病感染シュートにおける病徴発現の維持と多芽体を経由した病徴の消失
  • Sustained expression of dwarf symptoms in mulderry shoots grown in vitro and thir disappearance through the culture of multiple bud bodies

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Abstract

Multiple bud bodies (MBB) were induced by liquid culture from tips of in vitro grown shoots showing typical dwarf symptoms which were derived from dwarf diseased mulberry plants (Morus acidosa Griff.). The stock cultures of the diseased shoots did not lose the symptoms after 3 years of subculture and still contained phytoplasma in the vascular cells when observed under an electron microscope. There was no difference in morphology and growth rate between MBB induced from healthy and diseased shoots. The dwarf symptoms were reduced in some of the shoots regenerated from the MBB derived from the diseased shoots. The recovered shoots with reduced symptoms grew normally without expressing any apparent symptoms after acclimation and subsequent transfer to the field. Phytoplasma were not detected microscopically in the vascular cells of the shoot cultures that were established again from the field grown plant. Phytoplasma 16S rRNA gene was specifically amplified by PCR only in the stock cultures of diseased shoots. The present results showed that the proliferation of phytoplasma and expression of dwarf symptoms can be maintained stably for a long period of time in shoot cultures, and the pathogen and symptoms diminish or disappear through the induction of MBB and subsequent regeneration of shoots from the MBB.

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