Identification of insecticidal activity of Bacillus thuringiensis against the silkworm by DNA hybridization method.
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- ASANO SHINICHIRO
- Faculty of Agriculture, Hokkaido University
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- IIZUKA TOSHIHIKO
- Faculty of Agriculture, Hokkaido University
Bibliographic Information
- Other Title
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- DNAハイブリダイゼーション法によるBacillus thuringiensisの家蚕殺虫活性判定
- DNA ハイブリダイゼーションホウ ニ ヨル Bacillus thuring
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Abstract
In order to identify the insecticidal activity of 20 Bacillus thuringiensis subspecies which produce bipyramidal crystals, dot blot hybridization of DNA purified from the subspecies was performed with a cloned crystal protein gene (cryl-1 gene) from subsp. kurstaki HD-1. The DNAs from the ten subspecies exhibiting on insecticidal activity against the silkworm, Bombyx mori, strongly hybridized with the probe prepared from the cloned crystal protein gene. However, DNAs from the other ten subspecies lacking insecticidal activity did not hybridize with the probe. Similar results were also obtained in the experiment using a probe prepared from the DNA fragment that is specific for insecticidal activity. Based on these results, we concluded that the intensity of the insecticidal activity against the silkworm can be determined DNA hybridization.
Journal
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- The Journal of Sericultural Science of Japan
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The Journal of Sericultural Science of Japan 60 (6), 475-479, 1991
The Japanese Society of Sericultural Science
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Details 詳細情報について
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- CRID
- 1390001204529231488
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- NII Article ID
- 10011046070
- 130004031083
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- NII Book ID
- AN00190300
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- ISSN
- 1884796X
- 00372455
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- NDL BIB ID
- 3761395
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- Text Lang
- ja
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- Data Source
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- JaLC
- IRDB
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed