Quantitative analysis of several low molecular antibacterial substances in the digestive juice of 5th-instar silkworm larvae.

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  • ５齢期の家蚕消化液における低分子抗菌性物質の定量
  • 5レイキ ノ カサン ショウカエキ ニ オケル テイ ブンシ コウキンセイ ブ

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Caffeic acid (CA), protocatechuic acid (PA) and p-hydroxybenzoic acid (HA) which are low molecular antibacterial substances had been identified in the digestive juice of silkworm larvae by Koike et al. (1979). Quantity of CA which is the main antibacterial substance among the three substances is considered to play a significant role in the antibacterial activity of silkworms.<br>In this experiment, the content of CA in samples from 3 days old 5th-instar larvae and 5 days old larvae was found to be 250ppm and 440ppm, respectively. In larvae immediately after molting and in 7 days old ones, however, CA content was only 0.6 and 1.3ppm, respectively. In spite of the small quantities of CA, actual antibacterial activity was much higher than in the samples from 3 days old and 5 days old larvae. This finding suggests that low molecular antibacterial substances did not play an important role in these samples.

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