古田分離系ウイルスの化学的性状に関する研究

DOI 日本農学文献記事索引 Web Site

書誌事項

タイトル別名
  • Studies on the chemical properties of “flacherie virus isolated by Furuta” in the silkworm, <i>Bombyx mori</i>
  • フルタ ブンリケイ ウィルス ノ カガクテキ セイジョウ ニカンスルケンキュウ

この論文をさがす

説明

Chemical properties on a small flacherie virus, “flacherie virus isolated by Furuta” (FVF), were investigated and compared to those of Ina-flacherie virus (Bombyx-DNV). The purified FVF had a diameter of 22nm, and the bouyant density in CsCl was found to be 1.40. The nucleic acid in the intact virion was determined to be single-stranded DNA by diphenylamine reaction, formaldehyde reaction and acridine orange staining. Moreover, it was supposed that complementary single-stranded DNA (plus and minus) was contained in separate virions, since the DNA was extracted as double-stranded DNA under conditions of appropriate high-salt buffer and elevated temperature. Four structural proteins, one major protein (VP1) with a molecular weight (MW) of about 50, 000 and the other three minor proteins (VP2, VP3 and VP4) with MWs of about 57, 000, 70, 000 and 77, 000 were found in FVF by SDS-PAGE. These compositions were closely similar to those of Bombyx-DNV. Immunological investigation using Bombyx-DNV antiserum also showed that both FVF and Bombyx DNV are identical virus, or both are closely similar strain to each other.

収録刊行物

詳細情報 詳細情報について

問題の指摘

ページトップへ