Isolation and particular expression of a new .BETA.-tubulin gene in wing discs during metamorphosis of Bombyx mori.

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  • カイコの新しいβ-チューブリン遺伝子の単離と変態期の翅原基における特異的な発現
  • Isolation and particular expression of a new β-tubulin gene in wing discs during metamorphosis of Bombyx mori
  • Isolation and particular expression of

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Abstract

A new β-tubulin cDNA of Bombyx mori was isolated. Comparison of the deduced amino acid sequence with testis-specific Bombyx β-tubulin gave 79% identity. Northern analysis in wing discs showed that the β-tubulin gene encodes a 2.0kb mRNA whose expression was highest at S2 stage. Injection of 20-hydroxyecdysone (20E) increased the β-tubulin transcription in the wing disc. In situ hybridization revealed a lot of β-tubulin mRNA in the tracheoles at S2 stage. These results imply the possibility that the β-tubulin plays a role in the evagination of wing discs.

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