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Use of PCR with the specific primers for discrimination of <i>Nosema bombycis</i>
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- KAWAKAMI YUJI
- Laboratory of Environmental Biology, FCG Research Institute, Inc.
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- IWANO HIDETOSHI
- College of Bioresource Sciences, Nihon University
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- HATAKEYAMA YOSHINORI
- College of Bioresource Sciences, Nihon University Present address: Laboratory of Silkworm Pathology, Department of Sericulture, National Institute of Sericultural and Entomological Science
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- INOUE TADASHI
- College of Bioresource Sciences, Nihon University
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- CANNING ELIZABETH U.
- Imperial College at Silwood Park
Bibliographic Information
- Other Title
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- プライマーKAI01とKAI02を用いたPCRによる <i>Nosema bombycis</i> の識別
- プライマーKAI01とKAI02を用いたPCRによるNosema bombycisの識別
- Use of PCR with the specific primers for discrimination of Nosema bombycis
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Description
The specific amplification by PCR using a pair of primers, KAI01 and KAI02 (KAWAKAMI et al., 1995) was reinvestigated as to its ability to distinguish microsporidia belonging to genus Vairimorpha, Pleistophora and Nosema. DNA samples from microsporidia of genus Vairimorpha or Pleistophora so far tested, three species of Vairimorpha and one Pleistophora, failed to produce PCR products by those primers. Of Nosema spp., N. apis, N. furnacalis, N. mylittensis and Nosema tyriae from cinnabar moth, Tyria jacobeae did not also produce any product. Microsporidian parasites isolated from silkworms infected with pebrine in the northern part of India (courtesy of Dr. A. K. Bansal) and in southern India (courtesy of Dr. KIYOSHI KAWAKAMI) and in China (courtesy of Dr. X. CHEN) gave PCR products by KAI01 and KAI02 primers. The results from our study suggest that PCR tests with the use of KAI01 and KAI02 primers are useful to distinguish highly virulent strains of N. bombycis in pebrine inspection.
Journal
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- The Journal of Sericultural Science of Japan
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The Journal of Sericultural Science of Japan 70 (1), 43-48, 2001
The Japanese Society of Sericultural Science
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Details 詳細情報について
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- CRID
- 1390001204530833536
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- NII Article ID
- 10010268034
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- NII Book ID
- AN00190300
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- ISSN
- 1884796X
- 00372455
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- NDL BIB ID
- 5901034
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- Article Type
- journal article
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- Data Source
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- JaLC
- IRDB
- NDL Search
- CiNii Articles
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- Abstract License Flag
- Disallowed