Genetic study on alkaline phosphatases of the midgut tissue and digestive fluid of the silkworm, <i>Bombyx mori</i> L

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  • EGUCHI Masaharu
    Faculty of Textile Science, Kyoto University of Industrial Arts and Textile Fibers
  • YAMASHITA Yoshihiro
    Faculty of Textile Science, Kyoto University of Industrial Arts and Textile Fibers

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Other Title
  • カイコ幼虫の中腸組織と消化液のアルカリ性ホスファターゼについての遺伝学的研究
  • 家蚕幼虫の中腸組織と消化液のアルカリ性フォスファターゼについての遺伝学的研究
  • ケ カイコ ヨウチュウ ノ チュウチョウ ソシキ ト ショウカエキ ノ アルカ

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The genetic study on alkaline phosphatases of the midgut tissue and digestive fluid was carried out using two mutants. F (Aph1) and S (Aph2) isozymes of the midgut tissue in F2 generation of the cross of S- (phenotype F++S-) strain and F- (F-S++) strain were analysed by the polyacrylamide gel electrophoresis and the intensity of phosphatase bands was measured by a densitometer. From the result of segregation of F and S bands the recombination value between Aph1 and Aph2 was found to be 38.2%.<br>By comparison of the phosphatase activity in the digestive fluid and the intensity of F and S bands in F2 generaiton, the strong correlation of the S isozyme in the midgut tissue and digestive fluid phosphatase was shown.<br>The phosphatase activity in the digestive fluid of 494 larvae segregated in F2 generation was measured individually, and two peaks in frequency of phosphatase activity was found; the first peak is considered to correspond to Aph2-/Aph2-, and second peak corresponds to Aph2+/Aph2- and Aph2+/Aph2+ larvae.

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