Separation and Determination of Formaldehyde in Tanned Collagen by Gas-chromatography

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  • SATO Kyoji
    Leather Laboratory, Osaka Prefectural Industrial Research Institute
  • OKUMURA Akira
    Leather Laboratory, Osaka Prefectural Industrial Research Institute
  • KURATA Akio
    Leather Laboratory, Osaka Prefectural Industrial Research Institute
  • NAKAMURA Masashi
    Leather Laboratory, Osaka Prefectural Industrial Research Institute

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Other Title
  • ガスクロマトグラフィーによるなめしコラーゲン中のホルムアルデヒドの分離定量
  • ガス クロマトグラフィー ニヨル ナメシ コラーゲンチュウ ノ ホルムアルデヒ

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Description

The aldehydes are generally used for tanning agents. A modified method for assessing bound form aldehyde in tanned collagen by means of gas liquid chromatography (GLC) was described. The method consists of hydrolysis of collagen tanned with form aldehyde. conversion of the aldehyde into 2, 4-dinitrophenyl hydrazone (DNPH) and direct determination of DNPH by GLC. By this method, about 98% of formaldehyde bound to collagen can be determined.

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 50 (8), 369-371, 1976

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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