Studies on Comenic Acid and 5-Oxymaltol Accumulations by Microorganism isolated from Soil

ARIMA Kei, SAKURA Yuhei, IZAKI Kazuo

doi:10.1271/nogeikagaku1924.42.3_103

During the course of an investigation of the accumulation of maltol (3-hydroxy-2-methyl-γ-pyrone) by microorganisms, it was found that one strain No. 189, screened out from soil, accumulated two γ-pyrone compounds in the culture medium.<br> The present investigation was undertaken to clarify the progress of fermentation with the strain and to identify the two γ-pyrone compounds. No. 189 strain was gram-negative and motile by means of polar flagella (lophotrichous). The strain produced water-soluble yellow pigment and 2-keto gluconic acid in the culture medium. On the basis of these data, it seemed reasonable to assume that No. 189 strain was belonging to Pseudomonas.<br> The culture medium used contained 5% of sucrose, 1% of peptone, 0.1% of K₂HPO₄, 0.05% of MgSO₄ and 0.1% of yeast extract. Incubation was carried out for 15 days at 37°C using 500-ml shaking flask. In this fermentation, as 2-ketogluconic acid was produced, the organism could not grow after 7 days, but the two γ-pyrone compounds were accumulated 855μg/ml as kojic acid after 15 days.<br> In view of the fact that the two crystallized γ-pyrone compounds had identical IR absorption spectra and UV absorption spectra with authentic comenic acid and 5-oxymaltol, we concluded that one is comenic acid and the other is 5-oxymaltol.

Studies on Comenic Acid and 5-Oxymaltol Accumulations by Microorganism isolated from Soil

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