Studies on the Production of V. B<sub>12</sub> by Microörganism

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Other Title
  • 微生物によるビタミンB<sub>12</sub>生産に關する研究
  • ビセイブツ ニ ヨル ビタミン B12 セイサン ニ カンスル ケンキュウ 1
  • Part 1. The Production of V. B<sub>12</sub> by Propionibacterium. (1)
  • (第1報)プロピオン酸菌によるビタミンB<sub>12</sub>生産に就て (1)

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Description

We produced vitamin B12 by shaking culture of Propionibacterium freudenreich ATCC 6207 at. 30° and obtained 11 γ/cc vitamin B12 in the medium containing 2% lactic acid, 2% peptone, 1% dried yeast, 0.9% NaOH, and 1 p.p.m. CoCl2•6H2O.<br> We also obtained a good result by using the medium containing 2% glucose as carbon source, 2% peptone, 1% dried yeast, 0.8% K2HPO4, 1 p.p.m. CoCl2•6H2O on shaking machine (160 r. p. m., 6cm amplitude) and a maximum yield of Vitamin B12 was 1.8γ/cc on culture for 144 hours.<br> In the estimation of Vitamin B12 activity, the most simple microbiogical assay with E. coli 11105 was used together with the assay of Eugiena. Though the assay with E. coli has its weakpoint in sensibility and reliability, it seems to be complemented by using with the assay of Euglena.

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 27 (10), 680-684, 1953

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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