The Gas-chromatographic Separation of N-Trifluoroacetyl-dipeptide Methyl Esters

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  • N-TriHuoroacetyl-dipeptide methyl ester類のガスクロマトグラフによる分離
  • N-Trifluoroacethyl-dipeptide methyl esterルイ ノ ガスクロマトグラフ ニ ヨル ブンリ

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Description

It was ascertained that the N-trifluoroacetyl-dipeptide methyl esters were able to be separated efficiently by gas-chromatography using silicone grease column at 210° and a simple relation between the relative retention values and the structures of the compounds was observed. This method is helpful for the sequence analysis of the oligopeptide. According to the above method the gramicidine J1 was hydrolysed partially and the hydrolysate converted to N-trifluoroacetyl-methyl ester derivatives, which were then separated gaschromatographically. The five peaks of the dipeptide appeared on the chromatogram and they were identified as L-Pro-L-Val, L-Val-L-Orn, L-Orn-L-Leu, L-Leu-D-Phe and D-Phe -L-Pro. From this result it was concluded that the gramicidine J, has the same sequence as that of gramicidine S.

Journal

  • Nippon Nōgeikagaku Kaishi

    Nippon Nōgeikagaku Kaishi 39 (10), 391-396, 1965

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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