Effects of the cytidine analogue zebularine on wheat mitotic chromosomes

DOI Web Site 33 References
  • Cho Seong-Woo
    Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
  • Ishii Takayoshi
    Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
  • Matsumoto Naoya
    Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
  • Tanaka Hiroyuki
    Laboratory of Plant Genetics, Faculty of Agriculture, Tottori University
  • Eltayeb Amin Elsadig
    Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
  • Tsujimoto Hisashi
    Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University

Search this article

Abstract

Zebularine, cytidine analog is known as DNA methylation inhibitor such as 5-azacytidine and 5-aza-2'-deoxycytidine, and it is more stable in aqueous solution than 5-azacytidine and 5-aza-2'-deoxycytidine. We investigated effects of zebularine on plant mitotic chromosomes. A wheat disomic addition line carrying a pair of alien chromosomes (Leymus racemosus chromosome l) was treated with zebularine at various concentrations. The alien chromosomes were discriminated from the wheat chromosomes by genomic in situ hybridization, facilitating observation of any rearrangements between the wheat and alien chromosomes. Root growth was obviously inhibited by zebularine because of reduction of the mitotic division cells. Rearrangements such as ring chromosomes, insertions, deletions, and translocations were observed in the treated mitotic chromosomes. The aberrations were increased in a concentration-dependent manner of zebularine.

Journal

  • Chromosome Science

    Chromosome Science 14 (1+2), 23-28, 2011

    THE SOCIETY OF CHROMOSOME RESEARCH

References(33)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top