Effects of the cytidine analogue zebularine on wheat mitotic chromosomes
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- Cho Seong-Woo
- Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
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- Ishii Takayoshi
- Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
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- Matsumoto Naoya
- Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
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- Tanaka Hiroyuki
- Laboratory of Plant Genetics, Faculty of Agriculture, Tottori University
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- Eltayeb Amin Elsadig
- Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
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- Tsujimoto Hisashi
- Laboratory of Molecular Breeding, Arid Land Research Center, Tottori University
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Description
Zebularine, cytidine analog is known as DNA methylation inhibitor such as 5-azacytidine and 5-aza-2'-deoxycytidine, and it is more stable in aqueous solution than 5-azacytidine and 5-aza-2'-deoxycytidine. We investigated effects of zebularine on plant mitotic chromosomes. A wheat disomic addition line carrying a pair of alien chromosomes (Leymus racemosus chromosome l) was treated with zebularine at various concentrations. The alien chromosomes were discriminated from the wheat chromosomes by genomic in situ hybridization, facilitating observation of any rearrangements between the wheat and alien chromosomes. Root growth was obviously inhibited by zebularine because of reduction of the mitotic division cells. Rearrangements such as ring chromosomes, insertions, deletions, and translocations were observed in the treated mitotic chromosomes. The aberrations were increased in a concentration-dependent manner of zebularine.
Journal
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- Chromosome Science
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Chromosome Science 14 (1+2), 23-28, 2011
THE SOCIETY OF CHROMOSOME RESEARCH
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Details 詳細情報について
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- CRID
- 1390001204567616000
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- NII Article ID
- 10030317405
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- NII Book ID
- AA11163057
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- ISSN
- 21850852
- 13441051
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- NDL BIB ID
- 023607650
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed