An X-chromosome RNA-binding motif protein (RBMX) is required for proper kinetochore formation
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- Lee Mei Hann
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Lin Linyen
- Research Center for Ultra-High Voltage Electron Microscopy, Osaka University
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- Takata Hideaki
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Morimoto Akihiro
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Uchiyama Susumu
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Matsunaga Sachihiro
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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- Fukui Kiichi
- Department of Biotechnology, Graduate School of Engineering, Osaka University
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Description
RBMX is an RNA-binding motif protein identified as a component of purified human metaphase chromosomes. We have analyzed the functional significance of RBMX by electron microscopy (EM) in combination with RNA interference (RNAi). We revealed that depletion of RBMX results in the accumulation of mitotic cells. EM investigation further showed that RBMX depletion leads to an increase in the fuzzy ball structure that is reminiscent of the prekinetochore. It is most probably due to the result of disruption at early stages of kinetochore maturation, so that kinetochore differentiation into the authentic trilaminar morphology is disturbed. The present EM data illuminated the importance of RBMX in kinetochore formation for subsequent stable microtubules interaction.
Journal
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- Chromosome Science
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Chromosome Science 14 (1+2), 17-21, 2011
THE SOCIETY OF CHROMOSOME RESEARCH
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Keywords
Details 詳細情報について
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- CRID
- 1390001204567617664
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- NII Article ID
- 10030317380
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- NII Book ID
- AA11163057
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- COI
- 1:CAS:528:DC%2BC38Xps1Grsb0%3D
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- ISSN
- 21850852
- 13441051
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- NDL BIB ID
- 023607637
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- CiNii Articles
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- Abstract License Flag
- Disallowed