Detection of ribosomal RNA genes in apple (<i>Malus</i> × <i>domestica</i>) using fluorescence <i>in situ</i> hybridization
-
- Yamamoto Masashi
- Faculty of Agriculture, Kagoshima University
-
- Moriya Shigeki
- Division of Apple Research, NARO Institute of Fruit Tree and Tea Science
-
- Yamamoto Toshiya
- NARO Institute of Fruit Tree and Tea Science
Bibliographic Information
- Other Title
-
- Detection of ribosomal RNA genes in apple (Malus × domestica) using fluorescence in situ hybridization
Search this article
Abstract
<p>The locations of the 18S-5.8S-25S and 5S ribosomal RNA genes (rDNAs) on the chromosomes in the seedlings obtained from open-pollination of apple ‘Sensyu’ (Malus × domestica Borkh.) were determined using fluorescence in situ hybridization (FISH). 18S-5.8S-25S and 5S rDNA probes were labeled with biotin and hybridization signals were detected using a fluorescein isothiocyanate (FITC)-avidin conjugate on the chromosomes counterstained with DAPI. The 18S-5.8S-25S rDNA signals were detected in the telomeric positions of eight chromosomes among 34. These sites were located on two long, two relatively long, two medium, and two relatively short chromosomes. The two 5S rDNA sites were located at centromeric positions of relatively short chromosomes which do not possess 18S-5.8S-25S rDNA sites. The numbers and positions of rDNA sites were stable among the seedlings.</p>
Journal
-
- Chromosome Science
-
Chromosome Science 19 (1-4), 33-36, 2016
THE SOCIETY OF CHROMOSOME RESEARCH
- Tweet
Keywords
Details 詳細情報について
-
- CRID
- 1390001204568326784
-
- NII Article ID
- 130006837150
-
- NII Book ID
- AA11163057
-
- ISSN
- 21850852
- 13441051
-
- NDL BIB ID
- 028154605
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- CiNii Articles
-
- Abstract License Flag
- Disallowed