Removal of Bisphenol A by Peroxidase-Catalyzed Reaction Using Culture Broth of Coprinus cinereus

  • Sakurai Akihiko
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
  • Toyoda Satoko
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
  • Masuda Mina
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University
  • Sakakibara Mikio
    Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University

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説明

The removal of bisphenol A from aqueous solutions was examined using a culture broth of Coprinus cinereus as a source of peroxidase. Bisphenol A was efficiently removed by a polymerization and precipitation method catalyzed by peroxidase. The removal efficiency was optimal between pH 9–10 and at 40°C, and at a molar ratio of H2O2 to bisphenol A of about 2.0. To remove 100 mg/L bisphenol A, 3 U/mL peroxidase was required at pH 10 and 40°C, and 5 U/mL at pH 7 and 25°C. The acute toxicity of the bisphenol A solution, which was estimated using a bioluminescence method, decreased with the peroxidase treatment and became almost zero when the removal efficiency became 100%. Also, the estrogenic activity decreased due to the treatment.

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