Manipulation and Measurement of Single DNA Molecules.

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  • 一分子ＤＮＡの制御と計測
  • 解説 一分子DNAの制御と計測
  • カイセツ 1ブンシ DNA ノ セイギョ ト ケイソク

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Abstract

Manipulation and analytical method for single DNA molecules has been under development. The manipulation uses the transformation of DNA to globular state. Using the reverse transformation, single DNA molecules can be fixed in stretched mode for characterization. Fragments of the stretched DNA molecule can be cut and amplified by PCR (Polymerase Chain Reaction) using droplets as a container of single molecules and chemical agent. These techniques can be incorporated for micro-total analysis system.

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