分子拡散培養法を用いた培養心筋細胞の成長過程における自律拍動リズムの時間変化

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  • Developmental Changes in Spontaneous Beating Rhythm of Cardiac Myocytes in vitro Cultured with Molecular Diffusion Culture Method
  • ブンシ カクサン バイヨウホウ オ モチイタ バイヨウ シンキン サイボウ ノ セイチョウ カテイ ニ オケル ジリツ ハクドウ リズム ノ ジカン ヘンカ

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To understand the detailed mechanisms of arrhythmia and its treatment methods, it is required to elucidate the mechanisms of formation and debacle of cardiac beat at a microscopic level by using appropriate in vitro models of cardiac beating. In this study, we have developed a method for an medium-exchange-free culture of rat cardiac myocytes, and carried out long-term recording of electrical activity of cardiac myocytes from postnatal rat by means of microelectrode-array (MEA) and the new culture method. Figure 6 a) ∼ f) is a series of the properties of spontaneous beating (mean beating interval, standard deviation and coefficient of variation) of cardiac myocytes cultured by conventional culture method with alternate-day medium exchange (a ∼ c) or newly developed “molecular diffusion culture” method (d ∼ f). As shown in these figures, the difference of the beating property between the individual cultures, which is observed in conventional cultures (a) ∼ c)), was dramatically reduced in the molecular diffusion cultures (d) ∼ f)). In addition, these results also indicated that there is a transient period when all of mean beating interval, standard deviation, and coefficient of variation increase transiently before the formation of stable rhythmic fast beat.

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