書誌事項
- タイトル別名
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- Fabrication Process of Micro-patterns on Cell-Culturing Substrates toward High-Resolution Control and Observation of Cell Migration
- コウセイサイ ナ サイボウ ウンドウ セイギョ オヨビ カンサツ ニ ムケタ バイヨウ キバン エ ノ マイクロパターン ケイセイ シュホウ ノ カクリツ
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抄録
It is well known that cell migrations play a key role in the living systems. From the basic technical point of view, cell migration control is one of the important and useful ways to clarify the mechanism of the cell migration qualitatively and quantitatively. For the purpose of high-resolution control and observation of the cell migrations, which is the goal of this research, in this study, an attempt was made to establish the fabrication process of micro-patterns on caged cell-culturing substrates using inverted microscope with high magnification objective lens. With the combination of fluorescence antibody technique, the adequate ultraviolet (UV) lay exposure time was obtained for the formation of micro-patterns of fibronectin on the caged cell-culturing substrates. We also confirmed that it was possible to control the position of micro-patterns by the primary and secondary UV exposure. In addition, Swiss 3T3 cell culture experiment demonstrated that the initial shape of the cell could be restricted by the primary UV exposure and that the secondary UV exposure was remarkably useful to control the cell migration.
収録刊行物
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- 電気学会論文誌Ｃ（電子・情報・システム部門誌）
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電気学会論文誌Ｃ（電子・情報・システム部門誌） 131 (4), 833-839, 2011
一般社団法人 電気学会
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詳細情報 詳細情報について
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- CRID
- 1390001204608836608
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- NII論文ID
- 10027979966
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- NII書誌ID
- AN10065950
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- ISSN
- 13488155
- 03854221
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- NDL書誌ID
- 11065346
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可