Development of On-chip Multi-electrode Neural Cell Cultivation System using Thick Photoresist SU-8 and Semi-permeable Membrane for Long Term Studies

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  • 光硬化性樹脂SU-8と半透膜を用いた神経細胞長期培養用多電極刺激計測マイクロチャンバの試作
  • ヒカリ コウカセイ ジュシ SU 8 ト ハントウマク オ モチイタ シンケイ サイボウ チョウキ バイヨウヨウ タデンキョク シゲキ ケイソク マイクロチャンバ ノ シサク

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Abstract

A new type of multi-electrode array system for long term neural cell cultivation has been developed. The core of the system is an 8 room microchamber array, which consists of thick photoresist, SU-8, with 8-channel multi electrode array in those rooms. The biotin coated microchamber array plate is covered with the streptavidin decorated cellulose semipermeable membrane, which is fixed on the surface of the plate by streptavidin-biotin attachment, separating those rooms from the nutrient medium circulating above. The advantages of the system are; (i) contamination-free cultivation of neural cells achieved by the sealing of the semi-permeable membrane lid on the microchamber array, (ii) simple and flexible design of the microchamber array for position fixing of cells using the thick-photoresist wall with 1-5 micrometer width and 25 micrometer height, (iii) long-term simultaneous measurement of optical microscopic observation and electric recording. When nerve cells were cultured in the microchamber array, those cells have grown safely without any contamination. Those results proved a part of the potential of our multi-electrode array system.

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