Airborne Particulates: Cytotoxicity in HeLa Cells and the Induction of Sister Chromatid Exchanges in Chinese Hamster Don Cells

DOI
  • KURODA Koichi
    Osaka City Institute of Public Health and Environmental Sciences
  • HARUKI Kosuke
    Osaka City Institute of Public Health and Environmental Sciences
  • YOO Young S.
    Department of Preventive Medicine and Public Health Osaka City University
  • OKA Michio
    Osaka City Institute of Public Health and Environmental Sciences

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Other Title
  • 大気中粒子状物質の細胞毒性および姉妹染色分体交換誘起性

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Abstract

Benzene-extracts of airborne particulates (tars) were studied as to cytotoxic effect upon colony formation in HeLa cells. The dose-response relations of 4 tars were analyzed in concentrations of from 0 to 80 dug/mlat intervals of 10μg; 2 of them lowered colony formation to about 80% and the others to less than 50% at 40μg/ml. Although the latter toxic tars did not strongly reduce colony formation under 30 μg/ml, they did so logarithmically over 50μg/ml. These results show that the effects of tars on the colony formation in HeLa cells are in good agreement with the target theory. The extrapolated and D0 (intermediate lethal dose) values, the parameters of the target theory, were about 270% and 20μg/ml, respectively. The former weakly toxic tars showed gentle slopes under the same conditions.<BR>The ability of colony formation of tars collected from 8 monitoring stations in Osaka City were surveyed for about 2 years at 2 doses, 40 and 80μg/ml. The results showed that the cytotoxic effects of these tars at the identical time were very similar; local differences were not found. Seasonal differences, however, were observed. In the experimental period tars in each month, July to August and September to October in 1980, were found to be more cytotoxic in HeLa cells than the others; all of them inhibited completely colony formation at 80μg/ml.<BR>SCE-inductions of 6 tars collected from a station in an industrial area were also surveyed. A highly negative correlation was found between colony-forming rates and SCEs, suggesting that DNA in cells may be the target agent for cytotoxicity of tars.

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