Observation of motility and chemical reaction by single molecules of motor proteins
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- Higuchi Hideo
- Dept. Metallurgy and CIRTU, Tohoku Univ.
Bibliographic Information
- Other Title
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- 101生体分子モータの運動と化学反応を1分子で観察
- モーター タンパクシツ 1 ブンシ ノ ウゴキ オ ミル カガク ハンノウ モ ミル
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Description
The motilities of life are produced by motor proteins, for example, myosin molecule in muscle contraction, kinesin molecule in vesicle transportation. We reviewed the mechanics reaction and single ATP turnover of individual kinesin and myosin molecules. The displacement of kinesin during force generation bound to bead was determined by measuring the position of the beads with nanometer accuracy. The analysis of movement indicates that each kinesin head mainly steps by 16 urn between the binding sites while kinesin molecules exist at 8 nm intervals. The single ATP turnover was observed using fluorescence labeled ATP. The results suggest that myosin has a memory state which stores chemical energy from ATP hydrolysis.
Journal
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- Journal of the Visualization Society of Japan
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Journal of the Visualization Society of Japan 20 (1Supplement), 25-30, 2000
The Visualization Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204620866304
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- NII Article ID
- 10004570555
- 130003649186
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- NII Book ID
- AN10374478
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- ISSN
- 1884037X
- 09164731
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- NDL BIB ID
- 4954627
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed