A Simple Quantitative Measurement of mRNA of Human β-actin by Reverse Transcription Competitive PCR with a Compact Digital Camera
書誌事項
- タイトル別名
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- A Simple Quantitative Measurement of mRNA of Human .BETA.-actin by Reverse Transcription Competitive PCR with a Compact Digital Camera.
- Simple Quantitative Measurement of m RN
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抄録
Reverse transcription (RT) competitive polymerase chain reaction (PCR) is a sensitive and useful technique for the absolute quantitation of mRNA level. We developed a competitive PCR method with a compact digital camera DC 40 (Eastman Kodak Company, NY, U.S.A.) for the measurement of human β-actin cDNA (mRNA), a reference gene in an analysis for target gene expression. The devised method, compared with currently used methods, was easy, safe, sensitive, and highly reproducible to assay an infinitesimal level of cDNA (mRNA).
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 20 (9), 1013-1016, 1997
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204622994432
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- NII論文ID
- 110003639196
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 4307708
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- PubMed
- 9331988
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可