Production and Characterization of Monoclonal Antibodies against Two Haptenic Derivatives of 1α,25-Dihydroxyvitamin D3 Conjugated with Bovine Serum Albumin through the C-3 or C-24 Position
書誌事項
- タイトル別名
-
- Production and Characterization of Monoclonal Antibodies against Two Haptenic Derivatives of 1.ALPHA.,25-Dihydroxyvitamin D3 Conjugated with Bovine Serum Albumin through the C-3 or C-24 Position.
- Production and Characterization of Mono
この論文をさがす
抄録
To develop the immunochemical methods for determining 1α, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] in clinical samples, a variety of monoclonal anti-1, 25(OH)2D3 antibodies have been generated. Two kinds of hapten-carrier conjugates, 25-hydroxyvitamin D3 3-hemisuccinate (hapten 3-HS) and 1α-hydroxy-25, 26, 27-trinorvitamin D3 24-oic acid (hapten 24-OA) conjugated with bovine serum albumin, were used for immunization. Spleen cells from SD rats or BALB/c mice, each immunized with the conjugate of hapten 3-HS or 24-OA, were fused with P3/NS1/1-Ag4-1 myeloma cells. After screening by ELISA employing β-galactosidase-labeled haptens, seven kinds of hybridomas secreting anti-1, 25(OH)2D3 antibodies were established. Binding characteristics of these antibodies (Ka 0.73-20×109 M-1) were investigated by an RIA using tritium-labeled 1, 25(OH)2D3. The data suggested that the rat monoclonal antibody 3R-1 derived from the hapten 3-HS and the mouse monoclonal antibody 24M-3 from the hapten 24-OA would be available for developing practical analytical systems.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 20 (9), 948-953, 1997
公益社団法人 日本薬学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204623009024
-
- NII論文ID
- 110003639182
-
- NII書誌ID
- AA10885497
-
- COI
- 1:CAS:528:DyaK2sXmtl2qsro%3D
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 4307706
-
- PubMed
- 9331974
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可