Virus Concentration Using Sulfonated Magnetic Beads to Improve Sensitivity in Nucleic Acid Amplification Tests
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- Iwata Akiko
- The National Institute of Health Sciences, Japan
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- Satoh Koei
- The Institute of Saitama Red Cross Center
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- Murata Mitsuhiro
- JSR Corporation, Tsukuba Research Laboratories
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- Hikata Mikio
- JSR Corporation, Tsukuba Research Laboratories
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- Hayakawa Takao
- The National Institute of Health Sciences, Japan
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- Yamaguchi Teruhide
- The National Institute of Health Sciences, Japan
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説明
To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) and reverse-transcriptional (RT)-PCR, we developed a novel virus-concentration method using sulfonated (SO-) magnetic beads in the presence of divalent cations. In the presence of either Zn2+ or Cu2+ ions, we showed that SO-magnetic beads were able to concentrate non-enveloped model viruses, such as porcine parvovirus (PPV) and poliovirus, which were not concentrated by polyethyleneimine (PEI)-magnetic beads.1) Using the SO-magnetic beads, the sensitivity of virus genome detection by PCR or RT-PCR can be enhanced. Therefore, an efficient virus concentration method using either SO-magnetic beads or PEI-magnetic beads enhances the sensitivity of virus detection by PCR or RT-PCR.
収録刊行物
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- Ｂｉｏｌｏｇｉｃａｌ ＆ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｂｕｌｌｅｔｉｎ
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Ｂｉｏｌｏｇｉｃａｌ ＆ Ｐｈａｒｍａｃｅｕｔｉｃａｌ Ｂｕｌｌｅｔｉｎ 26 (8), 1065-1069, 2003
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204624422016
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- NII論文ID
- 110003655051
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BD3sznslWitw%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 6619919
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- PubMed
- 12913251
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDLサーチ
- Crossref
- PubMed
- CiNii Articles
- OpenAIRE
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- 抄録ライセンスフラグ
- 使用不可