Croton cajucara BENTH. Leaf Extract Scavenges the Stable Free Radical DPPH and Protects Against Oxidative Stress Induced by Paraquat
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- Tieppo Marcelo
- Laboratório de Estresse Oxidativo, Universidade Luterana do Brasil
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- Porawski Marilene
- Laboratório de Estresse Oxidativo, Universidade Luterana do Brasil
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- Salvador Mirian.
- Instituto de Biotecnologia, Universidade de Caxias do Sul
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- Moreira Andrea Janz
- Laboratório de Fisiologia Digestiva, Universidade Federal do Rio Grande do Sul, Brazil
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- Collado Pilar Sanzchez
- Department of Physiology, University of León
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- González-Gallego Javier
- Department of Physiology, University of León
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- Marroni Norma Possa
- Laboratório de Fisiologia Digestiva, Universidade Federal do Rio Grande do Sul, Brazil Laboratório de Estresse Oxidativo, Universidade Luterana do Brasil
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Antioxidant effects of extracts from Croton cajucara BENTH. leaves was investigated in different in vitro and in vivo models. Extracts showed inhibitory radical scavenging activity against the stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) (75%, 43% and 25% of the standard trolox at 1, 10 and 100 mg/ml, respectively; IC50 218 mg/ml). Percentage survival of Saccharomyces cerevisiae cells treated with 10 mM paraquat increased by 21% and 55%, when 1 mg/ml and 10 mg/ml concentrations of the extract, respectively, were added. The cytosolic concentration of TBARS increased in animals treated with paraquat (+283%), while values did not significantly differ from the controls in rats additionally receiving the leaf extract. Paraquat administration also induced a significant increase in hydroperoxide-initiated chemiluminiscence (+76%), that was partially prevented by the leaf extract (+31%). Liver SOD activity was a 158% higher in animals receiving paraquat as compared to the controls. This effect was abolished by administration of the leaf extract. Paraquat administration did not significantly modify the activity of GPx or catalase. Croton cajucara extract increased GPx and catalase activities in paraquat treated-animals by 342% and 70%, respectively. Our results confirm that Croton cajucara leaf extract present radical scavenging activity and reduce oxidative stress induced by paraquat, suggesting the beneficial use as a potential source of antioxidant agents of natural origin.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 29 (1), 161-165, 2006
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204624537856
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- NII論文ID
- 110005602104
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 7756174
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- 使用不可