Methyl-.BETA.-cyclodextrin Treatment Affects the Thermotropic Behaviour of Membranes and Detergent-Resistant Membrane Fractions of Cultured A431 Cells

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  • Masserini Massimo
    Department of Experimental, Environmental Medicine and Biotechnology, University of Milano-Bicocca
  • Pitto Marina
    Department of Experimental, Environmental Medicine and Biotechnology, University of Milano-Bicocca
  • Raimondo Francesca
    Department of Experimental, Environmental Medicine and Biotechnology, University of Milano-Bicocca
  • Cazzaniga Emanuela
    Department of Experimental, Environmental Medicine and Biotechnology, University of Milano-Bicocca
  • Sesana Silvia
    Department of Experimental, Environmental Medicine and Biotechnology, University of Milano-Bicocca
  • Bellini Tiziana
    Department of Biochemistry and Molecular Biology, University of Ferrara

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  • Methyl-β-cyclodextrin Treatment Affects the Thermotropic Behaviour of Membranes and Detergent-Resistant Membrane Fractions of Cultured A431 Cells
  • Methyl ベータ cyclodextrin Treatment Affects the Thermotropic Behaviour of Membranes and Detergent Resistant Membrane Fractions of Cultured A431 Cells

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Membranes and detergent-resistant membrane fractions isolated from human epidermoid carcinoma A431 cells after treatment with methyl-β-cyclodextrin, a compound commonly used in pharmaceutical applications and in manipulation of membrane cholesterol content, display thermotropic transitions at about 15 °C and above 37 °C, respectively, when analyzed by differential scanning calorimetry. The transitions, absent in untreated cells, were reversible upon cycling through heating and cooling scans, and attributable to lipid components of the membranes, possibly sphingolipids. These results suggest that, after treatment with methyl-β-cyclodextrin, membranes may show thermotropic transitions, an unusual feature for cellular bilayers, which is likely to influence biological functions.

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