Primary Structure and Properties of Ribonuclease Bm2 (RNase Bm2) from Bryopsis maxima
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- Itagaki Tadashi
- Department of Microbiology, College of Pharmacy, Nihon University
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- Koyama Hideki
- Department of Microbiology, College of Pharmacy, Nihon University
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- Daigo Satoshi
- Department of Microbiology, College of Pharmacy, Nihon University
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- Kobayashi Hiroko
- Department of Microbiology, College of Pharmacy, Nihon University
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- Koyama Takashi
- Department of Microbiology, College of Pharmacy, Nihon University
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- Iwama Masanori
- Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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- Ohgi Kazuko
- Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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- Irie Masachika
- Department of Applied Microbiology, Faculty of Pharmaceutical Sciences, Hoshi University
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- Inokuchi Norio
- Department of Microbiology, College of Pharmacy, Nihon University
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説明
A base non-specific ribonuclease (RNase Bm2) was isolated from a green algae (Ulvophyceae, Bryopsis maxima) as a single band on SDS-PAGE, and its primary structure and enzymatic properties, including base specificity, were investigated. The amino acid sequence of RNase Bm2 was homologous to many RNase T2 family RNases, and their characteristic CAS sequences were also conserved. The molecular mass of RNase Bm2 was 24444 Da, and its optimal pH was 5.5. RNase Bm2 was a poly U preferential RNase, similar to RNase MC1 from bitter gourd. The base specificity of this RNase suggested that the base specificity of the B1- and B2-base binding sites of RNase Bm2 were G≥U>C≫A and U>G>C≫A, respectively. The estimated active site of RNase Bm2 was very similar to that of RNase MC1 from bitter gourds; however, a tyrosine residue at the B1-base binding site that is conserved for all RNase T2 family RNases was replaced by a tryptophan residue. Here we discuss the effect of this replacement on the base specificity of RNase Bm2 and the phylogenetic relationship of RNase T2 family enzymes.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 29 (5), 875-883, 2006
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204624782464
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- NII論文ID
- 110005602207
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 7892258
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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