Caspase-3-Like Protease Activity-Independent Apoptosis at the Onset of Neuronal Cell Death in the Gerbil Hippocampus after Global Ischemia
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- Shimizu Hiroki
- Clinical Research Center, Eisai Co., Ltd.
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- Ohgoh Makoto
- Tsukuba Research Laboratories, Eisai Co., Ltd.
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- Ikeda Masuhiro
- Tsukuba Research Laboratories, Eisai Co., Ltd.
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- Nishizawa Yukio
- Tsukuba Research Laboratories, Eisai Co., Ltd.
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- Ogura Hiroo
- Tsukuba Research Laboratories, Eisai Co., Ltd.
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To investigate the relationship between caspase-3-like protease activity, which has been suggested to be related to apoptosis, and DNA fragmentation, we measured changes in caspase-3-like activity and DNA fragmentation in the hippocampus of gerbils exposed to global ischemia induced by bilateral occlusion of the carotid arteries for 5 min. Caspase-3-like protease activity began to increase at day 4 post-ischemia, reached a peak at day 5, and declined thereafter. The levels of DNA fragmentation, evaluated in terms of terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick-end labeling (TUNEL) staining and cytosolic nucleosomes, in the ischemic hippocampus began to increase significantly at day 3 after ischemia, reached a peak at day 4, and decreased thereafter. Our data suggest that DNA fragmentation in ischemic hippocampus of gerbils precedes caspase-3-like protease activation. Our results indicate that a caspase-3-like protease-independent apoptotic pathway operates, at least at the onset of neuronal cell death, in the hippocampus of gerbils after global ischemia.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 30 (10), 1950-1953, 2007
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204625152384
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- NII論文ID
- 110006436382
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 8919414
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可