Determination of Urinary Pyrraline by Solid-Phase Extraction and High Performance Liquid Chromatography.
-
- YOSHIHARA Kazuhiro
- Laboratory of Public Health, School of Pharmacy, Tokyo University of Pharmacy and Life Science
-
- KIYONAMI Reiko
- ThermoQuest K.K.
-
- SHIMIZU Yumi
- Laboratory of Public Health, School of Pharmacy, Tokyo University of Pharmacy and Life Science
-
- BEPPU Masatoshi
- Laboratory of Public Health, School of Pharmacy, Tokyo University of Pharmacy and Life Science
この論文をさがす
抄録
Pyrraline is one of the advanced glycation end products formed under non-enzymatic and non-oxidative conditions in vivo. In this study, we developed a novel method for determination of urinary pyrraline using solid-phase extraction as a pretreatment procedure prior to determination by high performance liquid chromatography (HPLC). The OasisTM HLB solid-phase extraction cartridge was used for pretreatment of urine samples without hydrolysis. The chromatogram obtained clearly revealed the peak for urinary pyrraline owing to prior removal of interfering substances in urine samples. The recovery rate of pyrraline was 97.2±3.3% (n=6). The mean excretion level of urinary pyrraline in healthy control (20—77 years old, n=30) was 1.42±0.65 μmol/mmol creatinine, and the daily variation in the excretion level was considered to be insignificant. We propose the above procedure as a simple, rapid, and accurate method for determination of pyrraline levels in urine.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 24 (8), 863-866, 2001
公益社団法人 日本薬学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204625193216
-
- NII論文ID
- 110003638577
-
- NII書誌ID
- AA10885497
-
- COI
- 1:CAS:528:DC%2BD3MXlvVCms7g%3D
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 5852061
-
- PubMed
- 11510474
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可