Apoptosis lnduced by Influenza Virus-Hemagglutinin Stimulation may be Related to Fluctuation of Cellular Oxidative Condition
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- Ohyama Kunio
- Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy & Life Science
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- Nishina Manabu
- Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy & Life Science
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- Yuan Bo
- Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy & Life Science
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- Bessho Toshio
- Yoneyama Maternity Hospital
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- Yamakawa Toshio
- Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy & Life Science
書誌事項
- タイトル別名
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- Apoptosis Induced by Influenza Virus-Hemagglutinin Stimulation may be Related to Fluctuation of Cellular Oxidative Condition.
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説明
Primary cultivated amnion epitherial cells prepared from amnion tissue of human fetal membrane (Amnion-cells) were stimulated with influenza virus-hemagglutinin (IV-HA), fractionated from a commercialized IV-HA vaccine by DEAE Sephacel column chromatography. From 72—96 h after stimulation, chromosomal DNA fragmentation and the appearance of in situ TUNEL stained-positive cells were revealed. Amnion-cell DNA fragmentation was inhibited in the presence of glycophorin A or C purified from the human erythrocyte membrane fraction, but not inhibited with free N-acetyl-neuraminic acid. RT-PCR and Western blotting analysis showed that anti-oxidative enzymes were altered with incubation period, accompanied by the expression of the cellular oxidative stress-related caspase cascade. Pre-stimulation of Amnion-cells with hemin, a heme oxygenase-1 inducer, significantly attenuated IV-HA induced DNA fragmentation. It is concluded that IV-HA induces apoptosis in Amnion-cells, and that this apoptotic induction may be facilitated by certain sialoglycoproteins on the cell surface, and is related to changes in the intracellular redox condition.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 26 (2), 141-147, 2003
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204625314688
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- NII論文ID
- 110003608392
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3sXjsFCqtrs%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 6418855
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- PubMed
- 12576671
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可