Morphine Inhibits Erythrocyte Carbonic Anhydrase in Vitro and in Vivo
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- Çoban Taha Abdulkadir
- Erzincan University, Erzincan Education Faculty, Department of Chemistry Education
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- Beydemir Şükrü
- Atatürk University, Science and Arts Faculty, Department of Chemistry
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- Gülçin İlhami
- Atatürk University, Science and Arts Faculty, Department of Chemistry
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- Ekinci Deniz
- Atatürk University, Science and Arts Faculty, Department of Chemistry
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説明
Morphine is implicated in diverse functions, from development to immune modulation in the central and peripheral nervous systems. At the present time, morphine is one of the most effective antinociceptive agents used to manage pain. It has been used extensively in the clinical management of pain due to its potent analgesic effect. In this study, the in vitro and in vivo inhibitory effects of morphine on erythrocyte carbonic anhydrase (CA) were investigated. Human erythrocyte isoenzymes, HCA-I and HCA-II, were purified by Sepharose-4B affinity chromatography column with a yield of 66.95 and 62.82%, a specific activity of 3892.3 and 11663.2 EU/mg proteins with 745.1 and 2232.6-fold purification of each isoenzyme, respectively. To determine enzyme purity, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed. In vitro inhibition of erythrocyte HCA-I and HCA-II by morphine using the CO2-hydratase enzyme gave IC50 values 4.50×10−5 M (r2: 0.954) and 9.23×10−5 M (r2: 0.996), respectively. CA activity was significantly attenuated in vivo in Spraque-Dawley rats for up to 3 h (p<0.001) following intraperitoneal administration of morphine. In conclusion, morphine inhibited CA activity both in vitro and in vivo.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 30 (12), 2257-2261, 2007
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204625474816
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- NII論文ID
- 110006546421
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 9274218
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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