In Vitro Metabolism of (-)-Camphor Using Human Liver Microsomes and CYP2A6
-
- Gyoubu Kunihiko
- Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University
-
- Miyazawa and Mitsuo
- Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University
この論文をさがす
抄録
The in vitro metabolism of (−)-camphor was examined in human liver microsomes and recombinant enzymes. Biotransformation of (−)-camphor was investigated by gas chromatography-mass spectrometry (GC-MS). (−)-Camphor was oxidized to 5-exo-hydroxyfenchone by human liver microsomal cytochrome (P450) enzymes. The formation of metabolites of (−)-camphor was determined by the relative abundance of mass fragments and retention time on gas chromatography (GC). CYP2A6 was the major enzyme involved in the hydroxylation of (−)-camphor by human liver microsomes, based on the following lines of evidence. First, of eleven recombinant human P450 enzymes tested, CYP2A6 catalyzed the oxidation of (−)-camphor. Second, oxidation of (−)-camphor was inhibited by (+)-menthofuran and anti-CYP2A6 antibody. Finally, there was a good correlation between CYP2A6 contents and (−)-camphor hydroxylation activities in liver microsomes of 9 human samples.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 30 (2), 230-233, 2007
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204625892224
-
- NII論文ID
- 110006162834
-
- NII書誌ID
- AA10885497
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 8616049
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可