Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells

  • Sekiya Nobuyasu
    Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University
  • Hikiami Hiroaki
    Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University
  • Yokoyama Koichi
    Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University
  • Kouta Kazufumi
    Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University
  • Sakakibara Iwao
    Pharmacognosy & Medicinal Resources Laboratory, Tsumura & Co.
  • Shimada Yutaka
    Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University 21st Century COE Program, Toyama Medical and Pharmaceutical University
  • Terasawa Katsutoshi
    21st Century COE Program, Toyama Medical and Pharmaceutical University

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Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochloride (AAPH)-induced hemolysis. The inhibitory effect was dose-dependent at concentrations of 10 to 1000 μg/ml. Moreover, tests were carried out to identify the main ingredient of ST that exerts a scavenging effect on free-radicals. Three representative alkaloids, tetrandrine, fangchinoline, and cyclanoline, isolated from ST, were found to have inhibitory activities against AAPH-induced lysis of red blood cells (RBC). Furthermore, the ingestion of 200 mg of ST extract was associated with a significant increase in free-radical scavenging effect of plasma in rats. These results suggest that ST as antioxidant inhibits AAPH-induced hemolysis of RBC both in vitro and in vivo.

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