Protective Effect of Moutan Cortex Extract on Acetaminophen-Induced Cytotoxicity in Human Chang Liver Cells.
-
- Shon Yun-Hee
- Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University
-
- Nam Kyung-Soo
- Department of Pharmacology, College of Medicine and Intractable Disease Research Center, Dongguk University
この論文をさがす
抄録
The aim of this study was to investigate the effect of Moutan Cortex on acetaminophen (AAP)-induced toxicity in human Chang liver cells. Cells were incubated with AAP (0—30 mM) to evaluate the drug’s ability to reduce cytoviability. For the cells treated with 10, 20 and 30 mM AAP, LDH leakage was 39.8%, 49.0% and 57.6%, respectively. Administration of Moutan Cortex reduced cytotoxicity in a dose-dependent manner. Glutathione (GSH) concentration in human liver cells decreased significantly after exposure to 20 (p<0.05) and 30 mM (p<0.01) AAP, and increased (p<0.05) if incubated with AAP and Moutan Cortex. The ability of AAP to inhibit mitochondrial function and its counteraction by Moutan Cortex was also evaluated. Moutan Cortex showed dose-dependent increases in MTT metabolism and ATP levels in AAP-treated cells. The DNA content of AAP-treated cells increased with the treatment of Moutan Cortex. These observations demonstrate that Moutan Cortex may significantly attenuate AAP-induced toxicity. It can be considered a cytoprotective agent in this in vitro model of drug toxicity.
収録刊行物
-
- Biological & Pharmaceutical Bulletin
-
Biological & Pharmaceutical Bulletin 25 (11), 1427-1431, 2002
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390001204626205440
-
- NII論文ID
- 110003608258
-
- NII書誌ID
- AA10885497
-
- COI
- 1:CAS:528:DC%2BD38XptlGlu7k%3D
-
- ISSN
- 13475215
- 09186158
-
- NDL書誌ID
- 6342366
-
- PubMed
- 12419953
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可