A Novel Method for Preparation of Animal Models of Liver Damage: Liver Targeting of Carbon Tetrachloride in Rats.
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- Mukai Takahiro
- School of Pharmaceutical Sciences, Nagasaki University
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- Mera Kunihiro
- School of Pharmaceutical Sciences, Nagasaki University
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- Nishida Koyo
- School of Pharmaceutical Sciences, Nagasaki University
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- Nakashima Mikiro
- Department of Hospital Pharmacy, Nagasaki University School of Medicine
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- Sasaki Hitoshi
- Department of Hospital Pharmacy, Nagasaki University School of Medicine
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- Sakaeda Toshiyuki
- Department of Hospital Pharmacy, School of Medicine, Kobe University
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- Nakamura Junzo
- School of Pharmaceutical Sciences, Nagasaki University
Bibliographic Information
- Other Title
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- Novel Method For Preparation of Animal Models of Liver Damage Liver Targeting of Carbon Tetrachloride in Rats
- A novel method for preparation of animal models of liver damage: liver trageting of carbon tetrachloride in rats
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Abstract
Animal models prepared by treatment with toxic compounds such as a carbon tetrachloride have been used to examine drug disposition in hepatic diseases. However, it is possible that these compounds accumulate and cause damage to other organs as they are administered systemically. In this study, we used the liver surface application technique to deliver a toxic compound to the liver to prepare an appropriate animal model in which only the liver is significantly damaged. To restrict the absorption area in the liver, a cylindrical diffusion cell was attached to the liver surface of male Wistar rats. Twenty-four hours after direct addition of carbon tetrachloride to the diffusion cell, plasma levels of glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT), and hepatic malondialdehyde (MDA) concentration were increased, while there were no changes in plasma creatinine or renal MDA level. On the other hand, not only GOT, GPT and hepatic MDA, but also creatinine and renal MDA levels were markedly increased by p.o. and i.p. administration of carbon tetrachloride, suggesting renal damage. These results indicated that the animal models of liver damage prepared by utilizing drug delivery techniques to accumulate toxic compounds in the liver would enable us to investigate the precise effects of hepatic disorder on drug disposition.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 25 (11), 1494-1497, 2002
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204626256384
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- NII Article ID
- 110003608274
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD38XptlGks70%3D
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- ISSN
- 13475215
- 09186158
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- HANDLE
- 10069/8379
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- NDL BIB ID
- 6342872
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- PubMed
- 12419969
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed