Pur .ALPHA. Protein Implicated in Dendritic RNA Transport Interacts with Ribosomes in Neuronal Cytoplasm.
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- LI Yanmei
- Department of Biochemistry, College of Pharmacy, Nihon University
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- KOIKE Katsuya
- Department of Biochemistry, College of Pharmacy, Nihon University
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- OHASHI Sachiyo
- Department of Biochemistry, College of Pharmacy, Nihon University
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- FUNAKOSHI Tomoko
- Department of Biochemistry, College of Pharmacy, Nihon University
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- TADANO Masahiro
- Department of Biochemistry, College of Pharmacy, Nihon University
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- KOBAYASHI Shunsuke
- Department of Biochemistry, College of Pharmacy, Nihon University
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- ANZAI Kaijiro
- Department of Biochemistry, College of Pharmacy, Nihon University
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- SHIBATA Noriyuki
- Department of Pathology, Tokyo Women’s Medical University
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- KOBAYASHI Makio
- Department of Pathology, Tokyo Women’s Medical University
Bibliographic Information
- Other Title
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- Pur α Protein Implicated in Dendritic RNA Transport Interacts with Ribosomes in Neuronal Cytoplasm
- Pur アルファ Protein Implicated in Dendritic RNA Transport Interacts with Ribosomes in Neuronal Cytoplasm
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Abstract
We have previously reported that pur α, known to be a regulator of DNA replication and transcription, links neural BC1 RNA to microtubules via dendrite-targeting RNA motifs. Here we demonstrate the subcellular localization of pur proteins within the brain. Pur proteins were detected in neurons but not in glia. Immunohistochemical staining was prominent in perikarya and proximal dendrites and also extended into primary dendritic processes, but no significant signals were detected in the distal regions of dendrite. When homogenates of mouse brain were fractionated, pur α was most concentrated in the microsomal pellet. Consistently, pur α co-fractionated with free polysomes as well as with membrane-bound polysomes and the association with polysomes was mediated by binding ribosomal subunits. Levels of ribosomes with pur α progressively increased during postnatal development of the brain.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 24 (3), 231-235, 2001
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204626440192
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- NII Article ID
- 110003663372
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXhs1Onuro%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 5682430
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- PubMed
- 11256476
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed