Accumulation of Medium Chain Acyl-CoAs during β-Oxidation of Long Chain Fatty Acid by Isolated Peroxisomes from Rat Liver

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  • HASHIMOTO Fumie
    Department of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Josai University
  • FURUYA Yasuko
    Department of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Josai University
  • HAYASHI Hidenori
    Department of Pathological Biochemistry, Faculty of Pharmaceutical Sciences, Josai University

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タイトル別名
  • Accumulation of Medium Chain Acyl-CoAs during .BETA.-Oxidation of Long Chain Fatty Acid by Isolated Peroxisomes from Rat Liver.
  • Accumulation of Medium Chain Acyl CoAs during ベータ Oxidation of Long Chain Fatty Acid by Isolated Peroxisomes from Rat Liver

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We have reported fatty alcohol synthesis accompanied by chain elongation in liver peroxisomes (Biochim. Biophys. Acta, 1346, 38 (1997)). In the present experiment, we studied what kind of acyl-CoA(s) destined to be utilized as primer for fatty alcohol synthesis accumulate(s) during peroxisomal β-oxidation. Peroxisomes were prepared from rat liver treated with clofibrate, a peroxisome proliferator, and incubated with [U-14C]palmitate, in order to investigate acyl-CoAs after β-oxidation. At 1 mM concentration, MgATP activated β-oxidation, but inhibited β-oxidation at concentrations higher than 1 mM. After incubation of peroxisomes with palmitate, various acyl-CoAs were formed. Among medium-chain labelled acyl-CoAs, octanoyl-CoA was mainly detected. There results suggest that octanoyl-CoA accumulates during β-oxidation of palmitate. When peroxisomes were incubated with [9,10-3H]palmitate and [9,10-3H]stearate, among medium-chain acyl-CoAs, octanoyl-CoA and decanoyl-CoA were primarily detected, respectively, suggesting the occurrence of at least 4 cycles of β-oxidation of both fatty acids by peroxisomes.

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