Effect of Delta-Tocotrienol on Melanin Content and Enzymes for Melanin Synthesis in Mouse Melanoma Cells
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- Michihara Akihiro
- Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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- Ogawa Saki
- Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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- Kamizaki Yohei
- Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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- Akasaki Kenji
- Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University
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In the present study, we investigated the dose-dependent effect of δ-tocotrienol long term (48, 72 h) on the melanin content of cells treated with δ-tocotrienol, and whether cells treated with δ-tocotrienol for long a time show cytotoxicity. We also examined whether other enzymes responsible for melanin biosynthesis, tyrosinase-related protein-1 (TRP-1) and -2 (TRP-2), are involved in the decrease in melanin levels. Protein levels in cells treated with 25 or 50 μM δ-tocotrienol for 48 h or 72 h were similar to those in control cells. Melanin content decreased by 44 (25 μM δ-tocotrienol) to 50% (50 μM) at 48 h, and by 14 to 21% at 72 h, compared to control levels. Tyrosinase activity, amounts of tyrosinase and TRP-1 decreased dependent on dose : by 50 (25 μM δ-tocotrienol) to 75% (50 μM), 20 to 45% and 42 to 82% at 48 h, and by 25 to 50%, 75 to 80% and 78 to 77% at 72 h, respectively. Although the amount of TRP-2 increased by 20% on treatment with 25 μM δ-tocotrienol for 48 h, it decreased by 52% on treatment with 50 μM δ-tocotrienol for 48 h. The amount of TRP-2 dose-dependently decreased by 55% and 75% on 72 h by treatment with 25 and 50 μM δ-tocotrienol, respectively. From these findings, δ-tocotrienol at up to 50 μM dose-dependently caused a reduction in melanin content by the decrease of TRP-1 and TRP-2 as well as tyrosinase, and no cytotoxicity.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 33 (9), 1471-1476, 2010
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204626544384
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- NII論文ID
- 130000322341
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 10796738
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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