Semi-vioxanthin isolated from marine-derived fungus regulates tumor necrosis factor-α, cluster of differentiation (CD) 80, CD86, and major histocompatibility complex class 2 expression in RAW264.7 cells via nuclear factor-κB and mitogen-activated protein kinase signaling pathways

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  • Yang Xin-Ying
    Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine
  • Cai Sheng-Xin
    Key Laboratory of Marine Drugs, Chinese Ministry of Education, Institute of Marine Drugs and Food, Ocean University of China
  • Zhang Wen-Ji
    Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine
  • Tang Xue-Lian
    Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine
  • Shin Hye-Young
    Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine
  • Lee Joo-Young
    Department of Life Science, Gwangju Institute of Science and Technology
  • Gu Qian-Qun
    Key Laboratory of Marine Drugs, Chinese Ministry of Education, Institute of Marine Drugs and Food, Ocean University of China
  • Park Hyun
    Department of Infection Biology, Zoonosis Research Center, Wonkwang University School of Medicine

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タイトル別名
  • Semi-vioxanthin Isolated from Marine-Derived Fungus Regulates Tumor Necrosis Factor-.ALPHA., Cluster of Differentiation (CD) 80, CD86, and Major Histocompatibility Complex Class II Expression in RAW264.7 Cells via Nuclear Factor-.KAPPA.B and Mitogen-Activated Protein Kinase Signaling Pathways
  • Semi vioxanthin isolated from marine derived fungus regulates tumor necrosis factor a cluster of differentiation CD 80 CD86 and major histocompatibility complex class 2 expression in RAW264 7 cells via nuclear factor kB and mitogen activated protein kinase signaling pathways

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Semi-vioxanthin isolated from marine-derived fungus was assessed for immunoregulatory activity in mouse RAW264.7 macrophages. In the present study, the facilitative effects of semi-vioxanthin on tumor necrosis factor-α (TNF-α) and its mRNA expression and on expression of the co-stimulatory molecules, cluster of differentiation (CD) 80, CD86 and major histocompatibility complex class II (MHC II), as well as the molecular mechanism underlying the immunologic enhancement properties of semi-vioxanthin were studied. Our results clearly indicated that semi-vioxanthin treatment resulted in the degradation of IκBα, which led to the activation and nuclear translocation of the p65 subunit of nuclear factor-κB (NF-κB), as determined by immunoblotting, immunofluorescence and electrophoretic mobility shift assays (EMSA). Moreover, TNF-α production was prevented by NF-κB and mitogen-activated protein kinase (MAPK) inhibitors. Inhibition of NF-κB and extracellular signal regulated kinases (ERK1/2) activity by specific inhibitors blunted the effect of semi-vioxanthin on the up-regulation of CD80, CD86 and MHCII expression, but neither p38 MAPK nor c-Jun N-terminal kinase (JNK) inhibitor had this effect. Thus, we demonstrate that semi-vioxanthin regulates TNF-α production through NF-κB and MAPK signaling pathways. Activation of NF-κB and ERK1/2 were necessary for CD80, CD86 and MHCII expression induced by semi-vioxanthin. These data suggest that semi-vioxanthin has immunoregulatory effects.

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