A Vanadyl Sulfate-Bovine Serum Albumin Complex Stimulates the Release of Lipoprotein Lipase Activity from Isolated Rat Fat Pads through an Increase in the Cellular Content of cAMP and myo-Inositol 1,4,5-Trisphosphate.
Bibliographic Information
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- A Vanadyl Sulfate-Bovine Serum Albumin Complex Stimulates the Release of Lipoprotein Lipase Activity Isolated Rat Fat Pads through an Increase in the Cellular Content of cAMP and myo-Inositol 1,4,5-Trisphosphate
- Vanadyl Sulfate-Bovine Serum Albumin Complex Stimulates the Release of Lipoprotein Lipase Activity Isolated Rat Fat Pads through an Increase in the Cellular Content of cAMP and myo-Inositol 1 4 5-Trisphosphate
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Description
A vanadyl sulfate-bovine serum albumin complex (vanadyl-BSA) prolonged the stability of the V4+ oxidation state, although vanadyl alone can readily change the oxidation state from V4+ to V5+ under physiological conditions. Vanadyl-BSA stimulated the release of lipoprotein lipase (LPL) acitivty from isolated rat fat pads and increased the cellular LPL activity in a time-dependent manner. These effects were independent of protein synthesis. Propranolol, quin 2-AM, ruthenium red, and neomycin all inhibited LPL release more potently than the increase in activity. In contrast, potent inhibition of the increase effect was observed with genistein and wortmannin. Short-term incubation of the fat pads with vanadyl-BSA showed a transient increase in the cellular content of cAMP and myo-inositol 1, 4, 5-triphosphate (IP3), which was inhibited by propranolol and neomycin, respectively. These results suggest that vanadyl-BSA stimulates the release of LPL activity through an increase in the cellular content of cAMP and IP3, leading to an increased intracellular Ca2+ concentration, and tat it also increases cellular LPL activity via process(es) sensitive to genistein and wortmanni.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 22 (8), 780-786, 1999
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204626618240
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- NII Article ID
- 110003639761
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- NII Book ID
- AA10885497
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- COI
- 1:CAS:528:DyaK1MXlsVOgurk%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 4834564
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- PubMed
- 10480313
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed