Phenolic Compounds from Artemisia iwayomogi and Their Effects on Osteoblastic MC3T3-E1 Cells

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One new (4) and twelve known phenolic compounds (1—3, 5—13) were isolated from a 70% MeOH extract of the aerial parts of Artemisia iwayomogi. The new compound was identified as 7,8-dimethoxy-coumarin-9-O-(6′-O-(E)-coumaroyl)-β-D-glucopyranoside (4) and named iwayomin. The effects of compounds 1—13 on the function of osteoblastic MC3T3-E1 cells were examined by evaluating cell viability, alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization in the presence of each compound. Compounds 3, 4, 7, and 9 showed potential in stimulating osteoblastic bone formation and may be useful for the prevention and/or treatment of osteoporosis.

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