Construction of Genomic DNA Library of Korean Ginseng (Panax ginseng C. A. MEYER) and Development of Sequence-Tagged Sites

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  • Bang Kyong-Hwan
    Ginseng Research Division, National Institute of Horticultural & Herbal Science, Korean Rural Development Administration (RDA)
  • Lee Jei-Wan
    Ginseng Research Division, National Institute of Horticultural & Herbal Science, Korean Rural Development Administration (RDA)
  • Kim Young-Chang
    Ginseng Research Division, National Institute of Horticultural & Herbal Science, Korean Rural Development Administration (RDA)
  • Kim Dong-Hwi
    Ginseng Research Division, National Institute of Horticultural & Herbal Science, Korean Rural Development Administration (RDA)
  • Lee Eung-Ho
    Ginseng Research Division, National Institute of Horticultural & Herbal Science, Korean Rural Development Administration (RDA)
  • Jeung Ji-Ung
    Rice Research Division, National Institute of Crop Science, Korean Rural Development Administration (RDA)

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This study describes an efficient approach for developing sequence tagged sites (STS) for Panax ginseng C.A. MEYER, and their applications for line discrimination. By using the methylation filtering (MF) technique, a genomic library was constructed, in which clone inserts were derived from the hypomethylated regions of ginseng genome. A methylation unfiltered genomic library was also constructed and the clone inserts were compared to those from the MF library in terms of sequence characteristics. Sequence analysis revealed that MF efficiently enriched the protein coding region of P. ginseng, for which the repetitive DNA appeared to be as little as 2.5 fold lower than clones in the unfiltered library, and also indicated that the P. ginseng genome may contain a large fraction of methylated repetitive DNA elements. A total of 99 and 100 highly stringent STS primer sets were designed from the filtered and unfiltered library, respectively. Amplification products were tested for latent polymorphism across six cultivars of P. ginseng and other 2 Panax species using six endonucleases recognizing four-bases. STS primer sets described here will be useful for marker-assisted selection, genome mapping and line discrimination of P. ginseng or its cultivars from other Panax species.

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