Hypoxia-Inducible Factor-1.ALPHA. Is Involved in the Pro-angiogenic Effect of Hydrogen Sulfide under Hypoxic Stress
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- Liu XinHua
- Department of Pharmacology, School of Pharmacy and Institute of Biomedical Sciences, Fudan University
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- Pan LiLong
- Department of Pharmacology, School of Pharmacy and Institute of Biomedical Sciences, Fudan University
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- Zhuo Yang
- Department of Pharmacology, School of Pharmacy and Institute of Biomedical Sciences, Fudan University
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- Gong QiHai
- Department of Pharmacology, School of Pharmacy and Institute of Biomedical Sciences, Fudan University
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- Rose Peter
- Pharmaceutical Science Research Division, King's College, University of London
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- Zhu YiZhun
- Department of Pharmacology, School of Pharmacy and Institute of Biomedical Sciences, Fudan University
Bibliographic Information
- Other Title
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- Hypoxia-inducible factor-1α is involved in the pro-angiogenic effect of hydrogen sulfide under hypoxic stress
- Hypoxia inducible factor 1 a is involved in the pro angiogenic effect of hydrogen sulfide under hypoxic stress
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Abstract
Hydrogen sulfide (H2S) is known to have pro-angiogenic properties in mammals. In this study, we examined H2S played the role in pro-angiogenesis mediated by hypoxia-inducible factor (HIF)-1α under hypoxic conditions. Rat brain capillary endothelial cells (ECs) were treated with NaHS (a H2S donor) pretreated vascular smooth muscle cells (VSMCs) conditioned media. ECs proliferation was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ECs migration was assessed by chemotaxis chamber assay. Angiogenesis-associated gene expression levels were determined by reverse transcription-polymerase chain reaction (RT-PCR). HIF-1α and vascular endothelial growth factor (VEGF) accumulation was analyzed by Western blotting. HIF-1 binding activity was measured by electrophoretic mobility shift assay (EMSA). We found H2S induced both endothelial proliferation and migration in mimic hypoxic condition. In addition, H2S promoted VEGF and HIF-1α mRNA levels. H2S also significantly upregulated HIF-1α and VEGF protein levels and increased HIF-1α binding activity under hypoxic condition. Our findings suggest that HIF-1/VEGF is involved in H2S promotes proliferation and migration of ECs.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 33 (9), 1550-1554, 2010
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204626712960
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- NII Article ID
- 130000322355
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 10796930
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed