Metabolism of (<b>−</b>)-fenchone by CYP2A6 and CYP2B6 in human liver microsomes

DOI DOI Web Site Web Site Web Site ほか2件をすべて表示 一部だけ表示 被引用文献7件 参考文献25件 オープンアクセス
  • Miyazawa Mitsuo
    Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University
  • Gyoubu Kunihiko
    Department of Applied Chemistry, Faculty of Science and Engineering, Kinki University

書誌事項

タイトル別名
  • Metabolism of (+)-Fenchone by CYP2A6 and CYP2B6 in Human Liver Microsomes
  • Metabolism of (−)-fenchone by CYP2A6 and CYP2B6 in human liver microsomes

この論文をさがす

説明

The in vitro metabolism of (+)-fenchone was examined in human liver microsomes and recombinant enzymes. Biotransformation of (+)-fenchone was investigated by gas chromatography-mass spectrometry. (+)-Fenchone was found to be oxidized to 6-exo-hydroxyfenchone, 6-endo-hydroxyfenchone and 10-hydroxyfenchone by human liver microsomal P450 enzymes. The formation of metabolite of (+)-fenchone was determined by relative abundance of mass fragments and retention time with GC. CYP2A6 and CYP2B6 in human liver microsomes were major enzymes involved in the hydroxylation of (+)-fenchone, based on the following lines of evidence. First, of eleven recombinant human P450 enzymes tested, CYP2A6 and CYP2B6 catalyzed oxidation of (+)-fenchone. Second, oxidation of (+)-fenchone was inhibited by thioTEPA, (+)-menthofuran anti-CYP2A6 and anti-CYP2B6 antibodies. Finally, there was a good correlation between CYP2A6, CYP2B6 contents and (+)-fenchone hydroxylation activities in liver microsomes of 8 human samples.

収録刊行物

被引用文献 (7)*注記

もっと見る

参考文献 (25)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ